What Bacteria Are in Kefir? Why the Bottle Has No Strain List

Milk kefir grains on a terracotta dish, each one a small creamy-white cauliflower-like lump

“Which bacteria are in your kefir?” “Why are the strains not listed on the bottle?” We hear these two questions on LINE almost every week, and they are fair questions. Yogurt in the supermarket lists its cultures, and everyone says to check the strain code when buying probiotics. Kefir is different because nobody chooses its bacteria. It is fermented with kefir grains, a living community of bacteria and yeasts that is a little different in every kitchen in the world, and a little different in every batch.

The short answer

  • Kefir grains are alive. They look like small cauliflower florets and hold many kinds of bacteria and yeasts living together.
  • No two grains are the same. People have handed grains from one kitchen to the next for centuries, so every kitchen’s grains are a little different.
  • Every batch shifts a little with the season, the milk and the temperature.
  • So a strain test would describe one bottle only. We measure what holds for every bottle: in April 2024 SGS counted 57 billion live lactic acid bacteria in 100 g of our milk kefir, 57 times the national standard for fermented milk.

See the SGS reportAsk us on LINE

How we checked

  • Research: reviews and studies of kefir grains, read in six languages. Each one is linked beside the sentence it supports and listed at the end.
  • Our lab report: the SGS test of our milk kefir from April 2024.

What are kefir grains?

A kefir grain: a creamy white lump that looks like a small cauliflower floret

Try it: drag to turn the grain, tap it to look inside

This is a kefir grain. It is soft, springy and alive. The slippery coat is kefiran, a gel the bacteria make themselves.

A model, not a photo. Real microbes are far too small to see without a microscope; here they are drawn many times larger.

Many people hear “grains” and picture a powder from a laboratory. Kefir grains are something you can see and hold: soft, white, springy lumps that look like small florets of cauliflower.

Inside, it is crowded. Lactic acid bacteria, yeasts and a few acetic acid bacteria live together and pass food to each other. This way of living together is called symbiosis (Prado and colleagues, 2015).

They all sit in a soft gel called kefiran, which the bacteria make themselves. Kefiran wraps the whole community and holds it together (BC Centre for Disease Control, 2024). It is the slippery coat you feel on a healthy grain.

Where did kefir grains come from?

Nobody invented kefir grains, and nobody knows exactly how the first one formed (Katchieva and colleagues, 2025). Kefir comes from the Caucasus mountains, where families fermented milk in bags made of sheep or goat skin (Bourrie and colleagues, 2016).

Two stories are told there. In one, soft white lumps were found in the folds of a skin bag in which milk had soured again and again. In the other, the lumps grew on pieces of calf stomach kept in goat’s milk. Science has not settled it.

Kefir is certainly old: the DNA of a kefir bacterium was found in cheese about 3,600 years old from a desert cemetery in Xinjiang (Liu and colleagues, 2024; see our article on that cheese). As far as published reviews report, laboratories have not yet rebuilt a traditional grain from separate cultures. In practice, new grains grow from old grains.

Why are grains different all around the world?

Because kefir grains are handed on, never manufactured. Each line of grains has its own history: where it came from, which milk it was fed and how warm its kitchen was.

When researchers compared 25 kefir grains from 8 countries, almost every grain had the same leading bacteria, and the smaller members differed from grain to grain (Marsh and colleagues, 2013). A larger study of 64 grains from 25 countries found the same: grains did not sort by country (Walsh and colleagues, 2023). They differ from kitchen to kitchen, not from country to country.

The mix keeps moving at home, too. Taiwanese milk is richer in winter than in summer (Food Next, in Chinese), so the same grains make a slightly different kefir in January and in July.

Which microbes lead at which temperature?

The same grains grow a different mix of microbes in a cool kitchen and in a hot one. Move the temperature and see what studies found, and which species can grow there at all.

25 °C

What studies measured in kefir

At about 25 °C, round lactic acid bacteria lead

Round lactic acid bacteria, such as lactococci, outnumbered the rod-shaped ones about a thousand to one. Yeasts were plentiful, no acetic acid bacteria were found, and this kefir got the best taste score.

  1. Round lactic acid bacteriathe mostmeasured
  2. Rod-shaped lactic acid bacteriaa fewmeasured
  3. Yeastssomemeasured
  4. Acetic acid bacterianone foundmeasured

Which species can grow at this temperature?

From lab tests of each microbe on its own. In a grain they live together, so this shows who can be active, not who wins.

  • Lactococcus lactis
  • Lactococcus cremoris
  • Leuconostoc mesenteroides
  • Lactobacillus kefiranofaciens
  • Lactobacillus kefiri
  • Heat-loving lactic acid bacteria (only in some grains)
  • Kluyveromyces marxianus
  • Saccharomyces cerevisiae
  • Kazachstania unispora
  • Acetobacter

grows in lab testsunclear, or differs between strainsgrows best

Studies in kefir: Smirnova, 2014 (25 to 40 °C), Vorokh and Lekunovich (19 to 27 °C), Londero, 2012, Hecer, 2019 (4 °C), Satiyarti, 2026 (2 to 10 °C). They counted groups of microbes, not each species. Lab growth tests: BacDive culture collection, Georgalaki, 2021, Zheng, 2020, Hamasaki, 2003, Salvadó, 2011, Kondybayev, 2023, Nonklang, 2008, Gomes, 2018. The drawing is a simplified picture.

Why is there no point in testing the strains?

A laboratory can read the genes in a sample and name the microbes it finds. This is called sequencing. It suits a product made from a few chosen strains, because every pack holds the same ones. With kefir grains it answers the wrong question.

  • One test describes one bottle. The next batch has the same groups in slightly different shares.
  • A strain code belongs to a culture that lives alone. A strain gets its name after a laboratory has separated it and grown it by itself; the guidelines also recommend storing it in a culture collection (FAO and WHO guidelines, 2002). In a kefir grain the microbes live mixed together.
  • It would tell you nothing new about your bottle. What counts is how many live bacteria it holds and that it is safe. Those two things we measure.

So we tell you what stays true for every bottle, and we keep away from names we could not promise for the next batch.

Yogurt and kefir: where is the difference?

Yogurt starts with a packet of powder, kefir with living grains. The international food standard draws the same line: yogurt is defined by two named bacteria, kefir by a culture made from kefir grains (Codex standard for fermented milks).

A packet of yogurt starter powder

Ordinary yogurt

Kefir grains on a wooden spoon

Kefir

Starts with

A packet of starter powder
Living kefir grains

What is inside

A few chosen lactic acid bacteria
A large community of bacteria and yeasts

Each batch

Always the same
A little different every time

On the pack

Each name can be listed
No fixed list to print

Afterwards

The powder is used up
The grains go into fresh milk again
A cup of plain yogurt
A jar of kefir with grains inside and an arrow showing the grains are used again

Species and strains are two different things

A species is a kind, like “mango”. A strain is one variety inside that kind, like Irwin and Jinhuang mangoes: both are mangoes, and they taste different.

A probiotic capsule is like a box of Irwin mangoes: a few chosen varieties, so each name and code can be printed. Kefir grains are like an old orchard with many kinds of fruit trees, where no two harvests are quite the same.

Which bacteria and yeasts do researchers find in kefir grains?

This list comes from published research on kefir grains from many countries. It is not a test of our own grains. We have not sequenced them.

How many kinds live in kefir? It depends on how you count.

  1. 7

    kinds grown in the lab from grains collected in Taiwan: 4 bacteria and 3 yeasts

    Chen, 2008; Wang, 2011

  2. 24–56

    kinds of bacteria in one grain, told apart by their DNA

    Marsh, 2013, counted by Bourrie, 2016

  3. 30–50

    species of bacteria and yeasts living together in one kefir

    Blasche, 2021

  4. 50+

    species found across all the grains studied so far

    Pogačić, 2013

  5. 861

    strains of 15 kinds of bacteria, in kefir made from 64 grains from 25 countries

    Walsh, 2023

in one grain or one kefiracross many grains

Growing microbes in the lab finds fewer kinds than reading their DNA, because many grow poorly alone. Inside one kefir, each species was usually present as one main strain (Walsh, 2023). The scale goes up in steps of ten.

Lactic acid bacteria make kefir sour and set the milk

  • Lactobacillus kefiranofaciens also in Taiwanese grains

    The main bacterium of the grain. It grows poorly on its own.

    Shape
    long rods
    Lives
    mostly in the grain
    Makes
    lactic acid and kefiran
    Taste
    linked to fruity notes
  • Lactobacillus kefiri also in Taiwanese grains

    Helps hold the grain together as a sticky film.

    Shape
    rods
    Lives
    grain and milk
    Makes
    lactic acid, a little acetic acid, gas
  • Lactococcus lactis also in Taiwanese grains

    Often the most common bacterium in the finished kefir, and one of the first to grow.

    Shape
    small balls in chains
    Lives
    mostly in the milk
    Makes
    lactic acid
  • Leuconostoc mesenteroides also in Taiwanese grains

    Often becomes the most common bacterium late in the fermentation.

    Shape
    round cells
    Lives
    mostly in the milk
    Makes
    acid and gas
    Taste
    buttery

Found in some grains: Lactobacillus helveticus, Lacticaseibacillus paracasei, Lactiplantibacillus plantarum, Lactobacillus acidophilus, Streptococcus thermophilus.

Yeasts make the fizz

  • Kluyveromyces marxianus also in Taiwanese grains

    The main yeast in the grains studied in Taiwan.

    Shape
    oval cells
    Milk sugar
    can use it
    Makes
    gas and a little alcohol
  • Saccharomyces cerevisiae

    The same species as baker’s yeast. It lives on what the bacteria leave.

    Shape
    oval, budding cells
    Milk sugar
    cannot use it
    Makes
    gas and a little alcohol
    Taste
    fruity, yeasty
  • Kazachstania unispora

    The most common yeast in grains from 8 countries.

    Shape
    oval cells
    Milk sugar
    cannot use it
  • Kazachstania turicensis also in Taiwanese grains

    Thought to help new grains form, by clumping together with Lactobacillus kefiranofaciens.

    Shape
    oval cells

Found in some grains: Kazachstania exigua, Pichia fermentans.

Acetic acid bacteria add a light vinegar-like tang

  • Acetobacter

    Common in grains, but missing from some.

    Shape
    short rods
    Makes
    acetic acid
    Taste
    vinegar-like

Found in some grains: Gluconobacter.

Bacteria people often ask about

  • Lacticaseibacillus rhamnosus: found in kefir grains in one study
  • Limosilactobacillus reuteri: one study
  • Bifidobacteria: only traces of DNA, never grown from kefir
  • Lactobacillus acidophilus, Streptococcus thermophilus, Lactobacillus bulgaricus: a few studies, in some grains

Sources: Bourrie, 2016, Prado, 2015, Georgalaki, 2021, Blasche, 2021, Walsh, 2016, Marsh, 2013, Codex and Wang, 2011.

What do we check?

The mix of microbes shifts a little from batch to batch. These are the things we measure.

  • How many live bacteria. In April 2024 SGS counted 57 billion live lactic acid bacteria in 100 g of our milk kefir. The national standard for fermented milk, CNS 3058, asks for at least 10 million per millilitre. Ours is 57 times that.
  • Safety. The same report tested for Salmonella, Listeria and the toxins of Staphylococcus aureus. All three were negative.
  • Every day. Our kefir is made in a professional food factory in Taiwan under strict hygiene control, and the acidity (pH) of every batch is measured.
SGS Taiwan test report AFA24404193 for our milk kefir: Salmonella, Listeria and Staphylococcus aureus enterotoxins negative, lactic acid bacteria 5.7 × 10⁸ CFU/g
SGS Taiwan report AFA24404193 on our milk kefir, 24 April 2024. English reference translation; the original report is in Chinese.

Looking for one particular strain?

Kefir is a food with a whole community, so it cannot promise one named strain. For that, a probiotic that prints the strain and its code on the pack can go alongside your daily glass of kefir.

See our milk kefirAsk us on LINE

Take the living grains home.

Living kefir grains can ferment fresh milk in your own kitchen too, batch after batch. They come with a printed care guide.

See live kefir grainsKefir grains 101

Questions customers ask us

Which bacteria are in kefir?

Mostly lactic acid bacteria and yeasts, with a few acetic acid bacteria. They live together in the kefir grains. The species researchers have named are in What researchers find.

Why does the bottle have no strain list?

Kefir is fermented by a whole living community, and its mix shifts a little with every batch. Yogurt uses a few fixed bacteria, so they can be listed one by one.

Does it contain Lactobacillus rhamnosus or another particular strain?

Kefir grains have their own bacteria, led by Lactobacillus kefiranofaciens. Bacteria known from capsules, such as Lacticaseibacillus rhamnosus, have been reported in kefir grains in a single study (Bourrie and colleagues, 2016). If you are looking for one particular strain, choose a product that prints that strain on the pack.

With no strain list, how do I know there are live bacteria in it?

In April 2024 SGS counted 57 billion live lactic acid bacteria in 100 g of our milk kefir. The soft hiss when you open the bottle and the fine fizz in the glass also show that the yeasts are still at work.

Kefir is thinner than yogurt. Does it have fewer bacteria?

No. How thick it is depends on the milk and the way it is fermented, not on how many bacteria it holds. The count our lab measured is in What we check.

Are kefir grains a powder made in a lab?

No. Kefir grains are soft, white, springy lumps that look like small cauliflower florets. The bacteria and yeasts live inside kefiran, a gel they make themselves. The grains grow in milk, and once strained out they go into fresh milk for the next batch. In practice, new grains grow only from existing grains. More in What kefir grains are.

Does the mix change while the bottle is in the fridge?

Only slowly. Cold slows the bacteria and yeasts right down, so the mix changes very little. The longer the bottle is kept, the more sour and fizzy the kefir gets. How temperature shifts the mix is shown in Temperature and who leads.

Any question? Just message us on LINE. We’re always happy to help.

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Research papers behind this article

Show all 21 papers

Milk kefir, fermented with living grainsSee it

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